Detalles del proyecto
Descripción
The overall project objective is to establish a foundational, systems-level understanding of plant, microbial, and environmental interactions that will lead to strategies for enhancing growth and sustainability of sorghum through genetic and microbial adaptations to water and nitrogen limited environments.
Project Description, Methods, and Outcomes: To compete in the biofuel energy market, cellulosic feedstocks will need to be high yielding and carbon neutral or negative while requiring low inputs. To avoid competition with existing food production systems, these crops will also need to be grown on marginal lands. This will require the introduction of novel traits to increase abiotic stresses tolerance associated with marginal soils. This project will utilize multiple interdisciplinary approaches in varied settings – including the laboratory, controlled environments, and the field – to identify plant genes and associated microbes that will enhance the sustainable production of sorghum as a biofuel feedstock. Basic knowledge about physiological and genetic mechanisms involved in nitrogen use efficiency (NUE) and water use efficiency (WUE) and potential mechanisms involved in microbe interactions will be generated. A range of methods will be used, including: classical whole plant physiology, stable isotope detection, phenomics, transcript profiling, metabolic profiling, 16S amplicon sequencing, metagenomics, microbial genome sequencing, comparative genomics, microbiology, genetics, and a range of computational methods for data analysis, integration and storage. To conduct these comprehensive studies, we have assembled a multi-institutional, interdisciplinary team with a wide range of expertise in these areas.
This research will increase our knowledge about the genetic and physiological mechanisms involved in WUE and NUE, which will be used to create sustainable sorghum biofuel feedstock systems on marginal land. Identification of microbial community membership and testing of culturable microbes, as well as genetic dissection of sorghum genotype X microbe interactions, will result in new strategies for the development of microbial solutions to increase abiotic stress tolerance and sustainable sorghum systems. Two major resources will be created: a sorghum microbe collection and a multi-dimensional relational database to house and access the biological materials and data generated in this project.
Estado | Finalizado |
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Fecha de inicio/Fecha fin | 15/8/15 → 14/8/22 |
Enlaces | https://pamspublic.science.energy.gov/WebPAMSExternal/Interface/Common/ViewPublicAbstract.aspx?rv=4ae51174-9eed-4df8-afb9-2d7ab1ea0efc&rtc=24&PRoleId=10 |
Financiación
- Biological and Environmental Research: USD13,460,684.00
!!!ASJC Scopus Subject Areas
- Botánica
- Edafología
- Biología molecular
- Fisiología
- Energía (todo)